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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in lots of tumors, which results in chromosome instability, and therefore could add to tumorigenesis. This protein was found to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a critical position in advertising and marketing mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually explained. Two extra splice variants have been described but in-depth nucleotide sequence information just isn't nevertheless readily available.
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Blocking peptides are peptides that bind exclusively for the goal antibody and block antibody binding. These peptide generally contains the epitope identified from the antibody.
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This manual outlines further possible optimization of cellular and immunoassay parameters to make sure the very best benefits are...
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This guide outlines even further achievable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies bound to the blocking peptide no longer bind towards the get more info epitope within the goal protein. By evaluating the staining in the blocked antibody as opposed to the antibody by itself, you can see which staining is unique.
Concentrate on Information and facts Cyclin E belongs towards the very conserved cyclin relatives, whose users are characterised by a extraordinary periodicity in protein abundance through the cell cycle. Cyclins purpose as regulators of CDK kinases. Distinctive cyclins show distinctive expression and degradation styles which add to the temporal coordination of each mitotic occasion. Cyclin E types a complex with and functions to be a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S changeover. This protein accumulates within the G1-S period boundary which is degraded as cells progress via S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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